Anti-γ-Tubulin antibody produced in rabbit
SIGMA/T5192 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-γ-Tubulin
Product Type: Chemical
Western Blotting JOURNAL CITATION: The exocyst protein Sec10 interacts with Polycystin-2 and knockdown causes PKD-phenotypes. By: Fogelgren, B., Lin, S. Y., et al. in PLoS Genet, 2011. PubMed ID: 21490950 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1001361), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium. By: Wang, W., Wu, T., et al. in Elife, 2014. PubMed ID: 25139956 Image collected and cropped by CiteAb from this publication, provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: The exocyst protein Sec10 interacts with Polycystin-2 and knockdown causes PKD-phenotypes. By: Fogelgren, B., Lin, S. Y., et al. in PLoS Genet, 2011. PubMed ID: 21490950 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1001361), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluorescence. CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:5,000 Anti-γ-Tubulin antibody produced in Rabbit (Cat. No. T3559) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 conjugate antibody, and 1:5,000 Anti-γA-Tubulin antibody produced in Rabbit (Cat. No. T5192) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate antibody. Results Two Anti-γ-Tubulin antibodies, T3559 (A) and T5192 (B), target different regions of γ-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence Centrosomal localization of γ-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 μg/mL Anti-γ-Tubulin (Cat. No. T5192), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 μg/mL Anti-γ-Tubulin (Cat. No. T5192), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in HeLa cells. Fixed cells were stained with 0.8 μg/mL Anti-γ-Tubulin (Cat. No. T5192), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in Rat2 cells. Fixed cells were stained with 0.8 μg/mL Anti-γ-Tubulin (Cat. No. T5192), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunoblotting Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 μg/mL Anti-γ-Tubulin (Cat. No.T5192), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| species reactivity | human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblasts cells |
| western blot: 1:1,000 using whole cell extract human epidermal carcinoma A431 cell line | |
| UniProt accession no. | P23258  |
| Application: | Anti-γ-Tubulin antibody produced in rabbit has been used in following studies: • Immunocytochemistry. • Immunofluorescence. • Immunoblotting. • Staining in microscopy. • Chromatin immunoprecipitation (ChIP). |
| Biochem/physiol Actions: | γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulinγ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule. γ-Tubulin does not polymerize with α-tubulin/β-tubulin, but instead it is localized to the centrosome and to the cytoplasm. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |