Monoclonal Anti-β-Tubulin antibody produced in mouse
SIGMA/T5293 - clone 2-28-33, ascites fluid
Synonym: Anti-Tubulin beta-1 chain
MDL Number: MFCD00145892
Product Type: Chemical
Western Blotting JOURNAL CITATION: Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction. By: Batiz, L. F., De Blas, G. A., et al. in PLoS One, 2009. PubMed ID: 19305511 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0004963), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-β-Tubulin (clone no. 2-28-33, Cat. No. T5293 and Goat Anti-Mouse IgG-FITC, F(ab′)2 fragment (Cat. No. F8521) and anti-centromere from human sera with Goat Anti-Human IgG-Cy3 (Cat. No. C2571). Chromosomes are counterstained with Hoechst (Cat. No. B2261). Images were acquired on a videomicroscope with a cooled CCD camera. From J. De Mey and P. Guillaud, Institute Jacques Monod, Paris.
Enhanced Validation-By Independent Antibodies Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:2,000 Monoclonal Anti-β-Tubulin antibody produced in Mouse, Clone: 2-28-33 (Cat. No. T5293) (A). The antibody was developed using 1:20 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262), and 1:400 Monoclonal Anti-β-Tubulin antibody produced in Mouse, Clone: TUB 2.1 (Cat. No. T4026) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-β-Tubulin antibodies, T5293 (A) and T4026 (B) target different regions of β-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting Whole cell extract of Chicken fibroblasts was separated on SDS-PAGE, and blotted with monoclonal Anti-ß Tubulin (Cat. No. T5293). The antibody was developed with Goat APA-Mouse Fab, Peroxidase conjugate (Cat. No. A9917) and AEC substrate. Lanes A: Antibody dilution 1:200 B: Negative control (only secondary antibody)
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2-28-33, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:1,000 using cultured chicken fibroblasts or cultured human foreskin fibroblasts |
| western blot: 1:100 | |
| UniProt accession no. | Q9H4B7  |
| Application: | Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and fluorescence immunocytochemical staining. |
| Biochem/physiol Actions: | Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins.Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). |
| Immunogen: | sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Specificity: | The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |