Transforming Growth Factor-β2 from porcine platelets
SIGMA/T5300 - powder, suitable for cell culture
Synonym: TGF-β2
MDL Number: MFCD00163957
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥97% (SDS-PAGE and N-terminal analysis) |
| biological source | Porcine (Platelets) |
| form | powder |
| impurities | endotoxin, tested |
| mol wt | protein 25 kDa |
| packaging | pkg of 1 μg |
| potency | 0.05-0.5 ng/mL ED50/EC50 |
| Quality Level | 200  |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | F1S9L0  |
| Analysis Note: | The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells. |
| Analysis Note: | The biological activity of porcine TGF-β2 was tested in culture by measuring its ability to inhibit thymidine incorporation in the IL-4 dependent mouse T-helper cell line HT-2. The EC50 is defined as the effective concentration of growth factor that elicits 50% inhibition of cell growth in a cell based bioassay. |
| Biochem/physiol Actions: | TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets. |
| Biochem/physiol Actions: | TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets. TGF-β2 belongs to the TGF-β superfamily. TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits NK cells growth as well as B and T cell proliferation. |
| Biochem/physiol Actions: | TGF-β2 is responsible for maintaining immune homeostasis. TGF-β2 promotes cellular growth, differentiation and survival. TGF-β2 controls lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 prevents NK (natural killer) cells growth as well as B and T cell proliferation. TGF-β2 also greatly inhibits the growth of both normal and transformed epithelial. The growth of lymphoid, fibroblast and keratinocyte cells are also known to be hinder by TGF-β2. TGF-β2 controls intestinal homeostasis by functioning in synergy with the bacterial lipopolysaccharide and thereby maintain interleukin-8. |
| General description: | TGF-β2 (transforming growth factor-β2) belongs to the TGF-β superfamily and the gene is mapped to pig (Sus scrofa) chromosome 10p16. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 25% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE and N-terminal analysis) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |