Anti-γ-Tubulin antibody, Mouse monoclonal
SIGMA/T5326 - clone GTU-88, purified from hybridoma cell culture
MDL Number: MFCD00677366
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Loss of primary cilia occurs early in breast cancer development. By: Menzl, I., Lebeau, L., et al. in Cilia, 2014. PubMed ID: 24987519 Image collected and cropped by CiteAb from the following publication, (http://ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-3-7), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Loss of primary cilia occurs early in breast cancer development. By: Menzl, I., Lebeau, L., et al. in Cilia, 2014. PubMed ID: 24987519 Image collected and cropped by CiteAb from the following publication, (http://ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-3-7), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear protein quality control centers during HSV-1 infection. By: Livingston, C. M., Ifrim, M. F., et al. in PLoS Pathog, 2009. PubMed ID: 19816571 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1000619), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Anti-γ-Tubulin (AK-15) antibody produced in Rabbit (Cat. No. T3320) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1 μg/mL Monoclonal Anti-γ-Tubulin antibody produced in Mouse, Clone: GTU-88 (Cat. No. T5326) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 Conjugate antibody. Results Two Anti-γ-Tubulin antibodies, T3320 (A) and T5326 (B) target different regions of γ-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2 μg/mL Monoclonal Anti-γ-Tubulin antibody produced in Mouse, Clone: GTU-88 (Cat. No. T5326) followed by Anti-Mouse IgG-Cy3 conjugate antibody.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 2 μg/mL Monoclonal Anti-γ-Tubulin Clone: GTU-88 (Cat. No. T5326). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate (red). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-γ-Tubulin Clone: GTU-88 (Cat. No. T5326). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. A431 3. COS7 4. NIH-3T3 5. Hepa1-6 6. PC-12 7. CHO 8. MDBK 9. MDCK
Immunoblotting Chicken fibroblast cells extract was separated on SDS-PAGE and probed with Monoclonal PA G-Tubulin (MO) CL:GTU-88 (Cat. No. T5326). The antibody was developed with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate (Cat. No. A1293) and an NBT/BCIP substrate. Antibody concentration 1 μg/mL.
| antibody form | purified antibody (purified from hybridoma cell culture) |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GTU-88, monoclonal |
| concentration | ~1 mg/mL |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 48 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, hamster, chicken, human, bovine, canine, Xenopus, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1-2 μg/mL using HeLa cells |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts | |
| UniProt accession no. | P23258  |
| Application: | Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies: • Western blot analysis • Immunohistochemistry • Immunocytology |
| Biochem/physiol Actions: | γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule. |
| Immunogen: | synthetic γ-tubulin peptide, conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | The antibody recognizes an epitope located within the N-terminal region of γ-tubulin. |
| Storage and Stability: | Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |