Anti-TTP (N-terminal) antibody produced in rabbit
SIGMA/T5327 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-G0/G1 switch regulatory protein 24; Anti-GOS24; Anti-Growth factor-inducible nuclear protein NUP475; Anti-NUP475; Anti-RNF162A; Anti-TIS11; Anti-Tristetraprolin; Anti-Zfp36, Zinc finger protein 36 homolog Protein
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition- Anti-TTP (N-terminal) antibody produced in Rabbit (Cat. No. T5327) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing TTP (Lanes 5-9) while no staining is detected in the wild type sample-HEK-293T (Lanes 1-4). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 0.5 MUg/mL. Lysates were treated as follows: Lanes 1-5: untreated control cell lysates. 2-3 and 6-8: cell lysates treated for 15 min at 37 °C with AP 4-7: cell lysates treated for 15 min at 37 °C with AP and Phosphatase inhibitors 9: cell lysate treated for 30 min at 37 °C with AP
Immunoblotting Lysae of RAW264 cells, induced for 2 hours with 10 ng/mL LPS, was separated on SDS-PAGE and probed with Rabbit Anti-TTP (N-terminal) (Cat. No. T5327). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 0.5 μg/mL 3. Antibody 1 μg/mL + 20 μg/mL TTP (N-terminal) immunizing peptide
Immunoblotting Detection of TTP multiple phosphorylation forms in HEK-293T cells over expressing human TTP. Cell extracts of untransfected (lanes 1-4) or TTP transfected (lanes 5-9) HEK-293T cells, were treated as indicated below with alkaline phosphatase (AP). Phosphatase inhibitors (Cat. Nos. P2850 and P5726) were added to the indicated lanes. The extracts were then separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-TTP (N-terminal) (Cat. No. T5327). The antibodies were developed using Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lysates were treated as follows: Lanes 1,5: untreated control cell lysates 2,3 and 6-8: cell lysates treated for 15 min at 37 °C with AP 4,7: cell lysates treated for 15 min at 37 °C with AP and Phosphatase inhibitors 9: cell lysate treated for 30 min at 37 °C with AP
Immunoprecipitation Rabbit Anti-TTP (N-terminal) (Cat. No. T5327) was used to immunoprecipitate human TTP from RAW264 cell lysates treated for 2 hours with 10 ng/mL LPS. Detection antibody: Anti-TTP (N-terminal) (Cat. No. T5327). Lanes 1. Immunobloting positive control: total lysate 2. IP antibody: 10 μg 3. Negative control: without cell lysate 4. Negative control: without IP antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 5-10 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours |
| western blot: 0.5-1.0 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours | |
| UniProt accession no. | P26651  |
| Application: | TTP (N-terminal) antibody produced in rabbit has been used in following studies: • RNA EMSA supershift • immunoblotting • ribonucleoprotein immunoprecipitation (RNP-IP) |
| Biochem/physiol Actions: | In activated monocytes and T lymphocytes, it regulates the expression of tumor necrosis factor a (TNF- α) by binding to a conserved AU rich element within the 3′ UTR of TNF-α mRNA. TTP is a phosphoprotein reported to be a substrate for kinases like MAPK activated protein kinase 2 (MAPKAPK2). The p38 signal transduction pathway is required for the expression of TTP protein and mRNA. |
| Biochem/physiol Actions: | Tristetraprolin (TTP) plays a key role in maintaining the stability of certain adenosine/uridine AU-rich element (ARE) mRNAs, by removing of the poly(A) tail and enhancing mRNA turnover. The encoded protein participates in mRNA binding and destabilization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tristetraprolin (TTP), also known as zinc-finger protein 36 (ZFP36), is a RNA-binding protein, encoded by the gene mapped to human chromosome 19q13.2. TTP is ubiquitously expressed and is characterized with a highly homologous RNA binding domain (RBD) with two CCCH zinc fingers (ZFs). |
| General description: | TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein TTP mRNA is widely distributed among tissue types, with a higher expression level in spleen, lymph nodes, and thymus. |
| Immunogen: | synthetic peptide corresponding to amino acids 51-67 of human TTP, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | TTP (N-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP belongs to a family of human |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |