apo-Transferrin human
SIGMA/T5391 - γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%
Synonym: Human transferrin; Siderophilin
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL Number: MFCD00130536
Product Type: Chemical
| assay | ≥98% |
| concentration | ~25 mM |
| form | powder |
| impurities | HIV and HBsAg, source material tested negative |
| iron, essentially free | |
| mol wt | 76-81 kDa |
| product line | BioXtra |
| Quality Level | 200  |
| shipped in | wet ice |
| solubility | H2O: 50 mg/mL |
| sterility | γ-irradiated |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02787  |
| Application: | apo-Transferrin human has been used as a supplement:• in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells • in DMEM/F-12 to culture neural precursor cells from mice • in collecting medium NCSU23 to culture preantral follicles from porcine ovaries |
| Biochem/physiol Actions: | apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells. |
| General description: | apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells. |
| Other Notes: | Non-heme iron-transport protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥98% |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |