Anti-Mouse IgG (whole molecule)−TRITC antibody produced in goat
SIGMA/T5393 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (whole molecule)
MDL Number: MFCD00162646
Product Type: Chemical
Peripheral C-cell-like neurons in a single hemisegment of a glossiphoniid leech embryo injected intracellularly with Lucifer yellow CH (Cat.No. L0259), background is the body-wall muscle labeled with Anti-Lan 3-14 and Goat Anti-Mouse IgG-TRITC (Cat. No. T 5393). From J. Jellies, Neurobiology Research Center, Univ. of Alabama, AL.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | TRITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:64 |
| Application: | Anti-Mouse IgG (whole molecule)-TRITC antibody may be used for immunofluorescence at a minimum antibody dilution of 1:64 using mouse spleen cells. Antibody dilution range of 1:50 to 1:250 was used for immunofluorescence in various studies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Antibody purification is by ion exchange chromatography after which it is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC). |
| General description: | Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. The antibody is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC. Specificity for mouse IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the γ region. |
| Immunogen: | IgG isolated from pooled normal mouse serum |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1, 2 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |