Transglutaminase from guinea pig liver
SIGMA/T5398 - lyophilized powder, ≥1.5 units/mg protein
Synonym: Protein-Glutamine-γ-Glutamyltransferase; Protein-
CAS Number: 80146-85-6
MDL Number: MFCD00132446
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| composition | Protein, ≥80% modified Warburg-Christian |
| form | lyophilized powder |
| mol wt | 76.6 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | H2O: soluble 1.0 mg/mL, clear |
| specific activity | ≥1.5 units/mg protein |
| storage temp. | −20°C |
| Application: | This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease. |
| Application: | Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity. |
| Biochem/physiol Actions: | Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme. |
| Physical form: | Lyophilized powder containing Tris and dithioerythritol |
| Unit Definition: | One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. ( |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1.5 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.3.2.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |