Transforming Growth Factor-β3 human
SIGMA/T5425 - TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture
Synonym: hTGF-β3; TGF-β3
MDL Number: MFCD00213054
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| form | powder |
| impurities | <0.01 EU/μg |
| mol wt | predicted mol wt ~25 kDa |
| packaging | pkg of 2 μg |
| potency | 0.01-0.09 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in baculovirus infected Sf21 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P10600  |
| Analysis Note: | The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells. |
| Application: | Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation. |
| Biochem/physiol Actions: | TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells. |
| Biochem/physiol Actions: | TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair. |
| General description: | The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |