Anti-TTP (C-terminal) antibody produced in rabbit
SIGMA/T5452 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-G0/G1 switch regulatory protein 24; Anti-GOS24; Anti-Growth factor-inducible nuclear protein NUP475; Anti-NUP475; Anti-RNF162A; Anti-TIS11; Anti-Tristetraprolin; Anti-Zfp36, Zinc finger protein 36 homolog Protein
Product Type: Chemical
Immunoblotting
Extract of HEK-293T cells over-expressing human TTP was separated on SDS-PAGE and probed with Rabbit Anti-TTP (C-terminal) (Cat. No. T5452). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody 1 μg/mL
2. Antibody 0.5 μg/mL
3. Antibody 1 μg/mL + 20 μg/mL TTP (N-terminal) immunizing peptide
Extract of HEK-293T cells over-expressing human TTP was separated on SDS-PAGE and probed with Rabbit Anti-TTP (C-terminal) (Cat. No. T5452). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody 1 μg/mL
2. Antibody 0.5 μg/mL
3. Antibody 1 μg/mL + 20 μg/mL TTP (N-terminal) immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using lysates of HEK-293 cells overexpressing human TTP |
| UniProt accession no. | P26651  |
| Application: | Anti-TTP (C-terminal) antibody has been used in immunoprecipitationand may be used for immunoblotting. |
| Biochem/physiol Actions: | Tristetraprolin (TTP) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP plays a role in both mRNA deadenylation and 3′ to 5′ degradation of the mRNA and helps in recruiting several factors involved in these processes. TTP acts as a substrate for multiple kinases like ERK (extracellular signal-regulated kinases), JNK (Jun N-terminal kinase) , p38, and MAPKAPK2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TTP is also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475. TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein that contains tandem CCCH zinc finger RNA-binding domains required for interaction with ARE. TTP is a phosphoprotein. Anti-TTP (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human TTP conjugated to KLH. |
| Immunogen: | synthetic peptide corresponding to amino acids 270-284 of human TTP conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | TTP (C-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |