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3,3′,5,5′-Tetramethylbenzidine

SIGMA/T5525 - tablet, 1 mg substrate per tablet

Synonym: BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate

CAS Number: 54827-17-7
Empirical Formula (Hill Notation): C16H20N2
Molecular Weight: 240.34
MDL Number: MFCD00007748
Linear Formula: [-C6H2(CH3)2-4-NH2]2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T5525-50TAB 50 tabs
 $246.00
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45-T5525-100TAB 100 tabs
 $420.00
1/EA		 Add To Favorites
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

 

form tablet
InChI 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key UAIUNKRWKOVEES-UHFFFAOYSA-N
mp 168-171 °C (lit.)
Quality Level 200 
SMILES string Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp. 2-8°C
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Application: 3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.
Hazard Codes Xi
Risk Statements 41
Safety Statements 26-39
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp 168-171 °C (lit.)
Storage Temp. 2-8°C
UNSPSC 12352204

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