TRAIL human
SIGMA/T5694 - recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder
Synonym: Apo2 Ligand; Apo2L; TL2; TNF-related apoptosis-inducing ligand
CAS Number: 2244730-93-4
MDL Number: MFCD01867158
Product Type: Chemical
| assay | >97% (SDS-PAGE) |
| biological source | human |
| description | N-terminal 6X histidine-tagged extracellular domain |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 24 kDa by SDS-PAGE |
| predicted mol wt ~21 kDa | |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P50591  |
| Analysis Note: | Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D. |
| Application: | TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line. |
| Biochem/physiol Actions: | TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells. |
| Biochem/physiol Actions: | Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |