Tricine
SIGMA/T5816 - BioPerformance Certified, suitable for cell culture, ≥99% (titration)
Synonym: 2-
CAS Number: 5704-04-1
Empirical Formula (Hill Notation): C6H13NO5
Molecular Weight: 179.17
EC Number: 227-193-6
MDL Number: MFCD00004277
Linear Formula: (HOCH2)3CNHCH2CO2H
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5816-100G
This image depicts SKU: T5816-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5816-500G
This image depicts SKU: T5816-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5816-1KG
This image depicts SKU: T5816-1KG
| absorption | ≤0.02 at 290 at 20% |
| application(s) | diagnostic assay manufacturing general analytical life science and biopharma sample preparation |
| assay | ≥99% (titration) |
| cation traces | heavy metals (as Pb): ≤5 ppm |
| color | white |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | endotoxin and total aerobic microbial count, tested |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4- |
| InChI key | SEQKRHFRPICQDD-UHFFFAOYSA |
| mp | 187 °C |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | OCC(CO)(CO)NCC(O)=O |
| storage condition | dry at room temperature |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7.4-8.8 |
| Application: | Buffer component for separation of low molecular weight peptides. |
| Application: | Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography. |
| Features and Benefits: | • Suitable as a Buffer component, for Electrophoresis and Protein separation • Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C) • Tested for Endotoxins and Total Aerobic Microbial Count • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications |
| General description: | Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |
| mp | 187 °C |
| UNSPSC | 12161700 |