Tumor Necrosis Factor-α from rat
SIGMA/T5944 - TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: Cachectin; TNF-α
MDL Number: MFCD00282383
Product Type: Chemical
SDS-PAGE Tumor Necrosis Factor-α from Rat (Cat. No. T5994) was assessed on SDS-PAGE.
TNF alpha Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Bioassay for Tumor Necrosis Factor α The cytolytic activity of rat recombinant TNF-α (Cat. No. T5944) is measured using murine L929 cells in the presence of actinomycin D. The ED50 is defined as the effective concentration of growth factor that elicits 50% inhibition of cell growth.
| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | rat |
| color | white |
| form | powder |
| impurities | <1 EU/μgtested (LAL test) |
| mol wt | predicted mol wt ~17 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P16599  |
| Analysis Note: | The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D. |
| Application: | TNF-α influences the growth and function of both normal and neoplastic cells. TNF-α causes cytolysis or cytostasis of certain transformed cells being synergistic with γ-interferon in its cytotoxicity. |
| Application: | Tumor Necrosis Factor-α from rat has been used: • To stimulate inducible nitric oxide synthase (iNOS) expression in the macrophages as an indication of M1 macrophage activation • To study its effect on pancreatic β cell apoptosis • To evaluate the effect of progesterone on the expression of tumor necrosis factor (TNF)-α in synovial membrane • As a blocking antigen in the control, for immunohistochemical analysis |
| Biochem/physiol Actions: | Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. |
| General description: | The Tnf (tumor necrosis factor) gene is mapped to rat chromosome 20p12. |
| Physical form: | Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |