Anti-TSG101 (C-terminal) antibody produced in rabbit
SIGMA/T5951 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Tumor Susceptibility Gene 101
MDL Number: MFCD08705529
Product Type: Chemical
Immunoblotting
Cell lysates were separated on SDS-PAGE and blotted with Anti-TSG101 (N-terminal) (Cat. No. T5951). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:2,000
(Lane 1) A431; (Lane 2) NIH3T3; (Lane 3) Rat1; (Lane 4) A431 with inhibition of antibody using TSG101 immunizing peptide (10 μg/mL)
Cell lysates were separated on SDS-PAGE and blotted with Anti-TSG101 (N-terminal) (Cat. No. T5951). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:2,000
(Lane 1) A431; (Lane 2) NIH3T3; (Lane 3) Rat1; (Lane 4) A431 with inhibition of antibody using TSG101 immunizing peptide (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 46 kDa (additional band of ~105 kDa may be observed in some preparations) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 0.4-0.8 μg/mL using rat brain extract (S1 cytosolic fraction) |
| western blot: 1-2 μg/mL using cell lysates of mouse fibroblast NIH3T3 cells and human A431 cells | |
| UniProt accession no. | Q99816  |
| Application: | Anti-TSG101 (C-terminal) antibody has been used in immnunoblotting and western blotting. |
| Biochem/physiol Actions: | TSG101 mediates a variety of cellular functions including transcriptional regulation, ubiquitination, endosomal trafficking, proliferation, and cell survival. It has a central role in protein sorting during endocytosis. Human TSG101 is also required for the budding of several enveloped RNA viruses including human immunodeficiency (HIV) and Ebola viruses. TSG101 is involved in the regulation of the p53/MDM2 (proto-oncogene) pathway by affecting E3 ubiquitin-protein ligase MDM2 (MDM2) stability. The N-terminal UEV domain binds to a P(T/S)AP sequence of HIV-1 p6 Gag and Ebola Vp40 proteins required for budding. The proline-rich domain was found to act as an activation domain in transcriptional regulation. The C-terminal coiled-coil (CC2) domain was predicted to interact with the cell growth regulating protein stathmin and is involved in potential co-repressor activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tumor susceptibility gene 101 encodes a multi-domain binding protein TSG101 which is 46 kDa. It is expressed in all tissues throughout development and is essential for both embryonic development and normal cell growth. It has an N-terminal UEV (N-terminal ubiquitin E2 variant) domain. Subcellular localization of TSG101 fluctuates in a cell-cycle dependent manner being localized in the nucleus and Golgi complex during interphase and in mitotic spindles and centrosome during mitosis. |
| Immunogen: | synthetic peptide corresponding to amino acids 371-390 located at the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |