Trizma® base
SIGMA/T6066 - BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
absorption | ≤0.05 at 260 at 1 M |
≤0.05 at 290 at 40% | |
≤0.06 at 280 at 1 M | |
agency | meets EP testing specifications |
meets USP testing specifications | |
USP/NF | |
application(s) | diagnostic assay manufacturing |
assay | ≥99.9% (titration) |
bp | 219-220 °C/10 mmHg (lit.) |
description | aminopeptidase substrate |
form | crystalline powder |
grade | BioPerformance Certified |
impurities | DNase, RNase, protease, none detected |
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested | |
≤0.2% water (Karl Fischer) | |
InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
loss | ≤0.5% (loss on drying, 110 °C) |
mp | 167-172 °C (lit.) |
pH | 10.0-11.5 |
pKa (25 °C) | 8.1 |
Quality Level | 400 ![]() |
SMILES string | NC(CO)(CO)CO |
solubility | water: soluble (666 mg/ml, yielding a clear colorless solution) |
technique(s) | cell culture | mammalian: suitable |
useful pH range | 7-9 |
Application: | Trizma has been used: • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells • As a component of pre-hybridization buffer in in situ hybridization • To suspend dried protein before SDS-PAGE protein characterization • As a component of lysis buffer to wash and lyse cells before immunoprecipitation • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols |
General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Easily compare specifications for Trizma products with the Trizma specification table ![]() |
Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
Packaging: | 1 kg in poly bottle |
Packaging: | 100, 500 g in poly bottle |
Packaging: | 5 kg in poly drum |
Packaging: | T6066-1KT: Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.9% (titration) |
bp | 219-220 °C/10 mmHg (lit.) |
mp | 167-172 °C (lit.) |
UNSPSC | 12352104 |