Trizma® base
SIGMA/T6066 - BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-100G
This image depicts SKU: T6066-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-500G
This image depicts SKU: T6066-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-1KG
This image depicts SKU: T6066-1KG
| absorption | ≤0.05 at 260 at 1 M |
| ≤0.05 at 290 at 40% | |
| ≤0.06 at 280 at 1 M | |
| agency | meets EP testing specifications |
| meets USP testing specifications | |
| USP/NF | |
| application(s) | clinical research clinical testing diagnostic assay manufacturing |
| assay | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| color | white |
| description | aminopeptidase substrate |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | DNase, RNase, protease, none detected |
| Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested | |
| ≤0.2% water (Karl Fischer) | |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| loss | ≤0.5% (loss on drying, 110 °C) |
| mp | 167-172 °C (lit.) |
| pH | 10.0-11.5 |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: soluble (666 mg/ml, yielding a clear colorless solution) |
| storage condition | dry at room temperature |
| suitability | suitable for chromatography |
| suitable for immunoprecipitation | |
| suitable for molecular biology | |
| technique(s) | cell culture | mammalian: suitable |
| DNA extraction: suitable | |
| protein purification: suitable | |
| useful pH range | 7-9 |
| Application: | Trizma has been used: • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells • As a component of pre-hybridization buffer in in situ hybridization • To suspend dried protein before SDS-PAGE protein characterization • As a component of lysis buffer to wash and lyse cells before immunoprecipitation • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols • as a component of Tris/base solution in a sulforhodamine B (SRB) assay |
| Features and Benefits: | • Suitable for Cell culture, Biochemical and Molecular Biology Research • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C) • Tested for the presence of Anionic and Cationic traces • Tested for DNase, RNase, and protease |
| General description: | Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes. This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic. In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | T6066-1KT: Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| UNSPSC | 12352104 |