Trizma® base
SIGMA/T6066 - BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-100G
This image depicts SKU: T6066-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-500G
This image depicts SKU: T6066-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T6066-1KG
This image depicts SKU: T6066-1KG
| absorption | ≤0.05 at 260 at 1 M |
| ≤0.05 at 290 at 40% | |
| ≤0.06 at 280 at 1 M | |
| agency | meets EP testing specifications |
| meets USP testing specifications | |
| USP/NF | |
| application(s) | diagnostic assay manufacturing |
| assay | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| description | aminopeptidase substrate |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | DNase, RNase, protease, none detected |
| Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested | |
| ≤0.2% water (Karl Fischer) | |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| loss | ≤0.5% (loss on drying, 110 °C) |
| mp | 167-172 °C (lit.) |
| pH | 10.0-11.5 |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: soluble (666 mg/ml, yielding a clear colorless solution) |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7-9 |
| Application: | Trizma has been used: • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells • As a component of pre-hybridization buffer in in situ hybridization • To suspend dried protein before SDS-PAGE protein characterization • As a component of lysis buffer to wash and lyse cells before immunoprecipitation • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols |
| General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Easily compare specifications for Trizma products with the Trizma specification table . |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | T6066-1KT: Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| UNSPSC | 12352104 |