Anti-α-Tubulin antibody, Mouse monoclonal
SIGMA/T6074 - clone B-5-1-2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-α-Tubulin antibody produced in mouse; alpha-Tubulin
MDL Number: MFCD00145891
Product Type: Chemical
Western Blotting JOURNAL CITATION: Autophagy attenuates diabetic glomerular damage through protection of hyperglycemia-induced podocyte injury. By: Fang, L., Zhou, Y., et al. in PLoS One, 2013. PubMed ID: 23593240 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0060546), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: TgMORN1 is a key organizer for the basal complex of Toxoplasma gondii. By: Heaslip, A. T., Dzierszinski, F., et al. in PLoS Pathog, 2010. PubMed ID: 20140195 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1000754), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Autophagy attenuates diabetic glomerular damage through protection of hyperglycemia-induced podocyte injury. By: Fang, L., Zhou, Y., et al. in PLoS One, 2013. PubMed ID: 23593240 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0060546), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 μg/mL Anti-α-Tubulin antibody, Mouse monoclonal, Clone: DM1A (Cat. No. T6199) (A). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262), and 1 μg/mL Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: B-5-1-2 (Cat. No. T6074) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-α-Tubulin antibodies, T6199 (A) and T6074 (B) target different regions of α-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence CFB cells cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with1 μg/mL Monoclonal Anti-α-Tubulin antibody produced in mouse clone No. B-5-1-2 (Cat. No. T6074). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat (Cat. No. F5262). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence Monoclonal Anti-α-Tubulin antibody: Cat. No. T6074: Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and Anti-Mouse Alexa543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa488 secondary antibody. Nucleus seen thanks to DAPI staining. Confocal image. Submitted by Dr L. Francelle, Commissariat d′Energie Atomique.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-α-Tubulin, Clone: B-5-1-2 (Cat. No. T6074). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. JURKAT; 3. COS7; 4. NIH-3T3; 5. PC-12; 6. RAT2; 7. CHO; 8. MDBK; 9. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | B-5-1-2, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB) |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using total cell extract of human foreskin fibroblast cell line (FS11) | |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in immunoblotting. |
| Biochem/physiol Actions: | Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS). α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS). Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α -tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α -subunit of tubulin has molecular weight of 50,000. Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the hybridoma B-5-1-2 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Both subunits have a molecular weight of approx. 50 kDa and share considerable homology. In addition to α- and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven. Most of these tubulins have distinct subcellular localization and an emerging diverse set of functions. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Microtubular systems contain at least three α-tubulin isoforms. Two isoforms are coded by two αt-ubulin genes, which are both transcribed and code for extremely similar proteins. The third isoform is generated by post-translational modification. |
| Immunogen: | Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |