Trypan Blue
SIGMA/T6146 - powder, BioReagent, suitable for cell culture
Synonym: Direct blue 14; Niagara blue reagent; blue live/dead cell assay; Direct blue 14
CAS Number: 72-57-1
Empirical Formula (Hill Notation): C34H24N6Na4O14S4
Molecular Weight: 960.81
EC Number: 200-786-7
MDL Number: MFCD00003969
Linear Formula: C34H24N6O14S4Na4
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T6146-100G
| application(s) | cell analysis |
| composition | Dye content, ~40% |
| form | powder |
| InChI | 1S/C34H28N6O14S4.4Na/c1-1 |
| InChI key | GLNADSQYFUSGOU-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| packaging | glass bottle of 100 g |
| glass bottle of 25 g | |
| glass bottle of 5 g | |
| product line | BioReagent |
| Quality Level | 300  |
| SMILES string | [Na+].[Na+].[Na+].[Na+].C |
| solubility | H2O: 10 mg/mL |
| storage condition | dry at room temperature |
| technique(s) | cell culture | mammalian: suitable |
| tissue processing: suitable |
| Application: | Trypan blue has been used: • in cell viability assay • to stain plant tissue in order to to visualize cell death |
| Biochem/physiol Actions: | The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm. |
| General description: | The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol. |
| Packaging: | 5, 25, 100 g in glass bottle |
| Preparation Note: | To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H350 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 45 |
| Safety Statements | 53-45 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| mp | >300 °C (lit.) |
| Colour Index Number | 23850 |
| UNSPSC | 12352207 |