Trypan Blue

SIGMA/T6146 - powder, BioReagent, suitable for cell culture

Synonym: Direct blue 14; Niagara blue reagent; blue live/dead cell assay; Direct blue 14

CAS Number: 72-57-1
Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.81
EC Number: 200-786-7
MDL Number: MFCD00003969
Linear Formula: C₃₄H₂₄N₆O₁₄S₄Na₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T6146-5G	5 g	$23.10 1/EA	Add To Favorites
45-T6146-25G	25 g	$38.80 1/EA	Add To Favorites
45-T6146-100G	100 g	$111.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T6146-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	cell analysis
composition	Dye content, ~40%
form	powder
InChI	1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key	GLNADSQYFUSGOU-UHFFFAOYSA-J
mp	>300 °C (lit.)
packaging	glass bottle of 100 g
	glass bottle of 25 g
	glass bottle of 5 g
product line	BioReagent
Quality Level	300
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
solubility	H₂O: 10 mg/mL
storage condition	dry at room temperature
technique(s)	cell culture \| mammalian: suitable
	tissue processing: suitable

Application:	Trypan blue has been used: • in cell viability assay • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution. • to stain plant tissue in order to to visualize cell death
Biochem/physiol Actions:	The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
General description:	The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.
Packaging:	5, 25, 100 g in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	45
Safety Statements	53-45
RIDADR	UN 3077 9 / PGIII
WGK Germany	WGK 3

mp	>300 °C (lit.)
Colour Index Number	23850
UNSPSC	12352200

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