Triosephosphate Isomerase from rabbit muscle
SIGMA/T6258 - Type X, lyophilized powder, ≥3,500 units/mg protein
Synonym: D-
CAS Number: 9023-78-3
EC Number: 232-774-2
MDL Number: MFCD00082080
Product Type: Chemical
| composition | Protein, 60-90% biuret |
| foreign activity | pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphat |
| form | lyophilized powder |
| mol wt | calculated mol wt 53.2 kDa |
| Quality Level | 200  |
| specific activity | ≥3,500 units/mg protein |
| storage temp. | −20°C |
| type | Type X |
| Application: | Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS). |
| Application: | Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure. |
| Biochem/physiol Actions: | Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease. |
| General description: | Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state. |
| Packaging: | 1, 5 mg in serum bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Sulfate-free, contains EDTA and borate buffer salts |
| Unit Definition: | One unit will convert 1.0 μmole |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3,500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 5.3.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |