Monoclonal Anti-Troponin T antibody produced in mouse
SIGMA/T6277 - clone JLT-12, ascites fluid
Synonym: Anti-ANM; Anti-NEM5; Anti-STNT; Anti-TNT; Anti-TNTS
MDL Number: MFCD00164752
Product Type: Chemical
Immunofluorescence
Staining of a formaldehyde-fixed longitudinal section of human myocardium using Monoclonal Anti-Troponin T, clone JLT-12, (Cat. No. T6277). Typical cross-striation is observed corresponding to the I bands in all cardiomyocytes. Nuclei are stained red with propidium iodide. Yellow fluorescence is caused by lipofuscin granules. The extracellular space is dark and shows only nuclei of fibroblasts stained red.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
Staining of a formaldehyde-fixed longitudinal section of human myocardium using Monoclonal Anti-Troponin T, clone JLT-12, (Cat. No. T6277). Typical cross-striation is observed corresponding to the I bands in all cardiomyocytes. Nuclei are stained red with propidium iodide. Yellow fluorescence is caused by lipofuscin granules. The extracellular space is dark and shows only nuclei of fibroblasts stained red.
From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
Western Blotting Whole extract of Rabbit leg skeletal muscle were separated on SDS-PAGE and probed with Monoclonal Anti-Troponin T antibody produced in Mouse, Clone: JLT-12 (Cat. No. T6277). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:200 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | JLT-12, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, bovine, rabbit |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200 using rabbit total skeletal muscle extract | |
| UniProt accession no. | P13805  |
| Application: | Monoclonal Anti-Troponin T antibody produced in mouse has been used in following studies: • Western blot analysis. • Immunocytochemical analysis. • Immunofluorescence. |
| Application: | Monoclonal Anti-Troponin T antibody produced in mouse has been used in immunostaining. |
| Biochem/physiol Actions: | Troponin T is a subunit of troponin, which facilitates binding of troponin to tropomyosin. It also plays a crucial role in muscle contraction. Cardiac troponin T acts as a potential marker for the detection of acute myocardial infarction (MI). Apart from facilitating binding of troponin components to the actin-tropomyosin filament, it is has an essential role in the regulation of actomyosin ATPase activity. Cardiac troponin T gene mutation leads to hypertrophic cardiomyopathy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Troponin T (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | Troponin T is a vital subunit of troponin. Troponin T is a comma or rod shaped protein with a length of 185-205 Å. Troponin T is positioned in the actin helix groove and is extended along the filament of troponin. Life span of serum troponin T is 120 minutes. N- terminal end of the protein is enriched in negatively charged residues and C- terminal end is enriched with positively charged residues. This uneven distribution of charges in troponin T facilitates its aggregation at physiological salt concentration. In humans, cardiac troponin T exists in four isoforms, out of which three are expressed in the fetus and one isoform is expressed in the adult heart. |
| Immunogen: | Troponin T from rabbit skeletal muscle. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |