Anti-TOM22 antibody , Mouse monoclonal
SIGMA/T6319 - clone 1C9-2, purified from hybridoma cell culture
Synonym: Anti-Mitochondrial import receptor Tom22; Anti-TOMM22; Anti-Translocase of outer mitochondrial membrane 22 homolog (yeast)
MDL Number: MFCD03457578
Product Type: Chemical
Western Blotting Whole extract of cultured Human lymphoma Raji cells were separated on SDS-PAGE and probed with Anti-TOM22 antibody, Mouse Monoclonal, Clone: 1C9-2 (Cat. No. T6319). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C9-2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 22 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rodent |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using whole extract of cultured human lymphoma Raji cells. | |
| UniProt accession no. | Q9NS69  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) • Western blotting |
| Biochem/physiol Actions: | Translocase of outer mitochondrial membrane 22 (TOMM22) is a key constituent of TOM complex. It functions as a mitochondrial receptor for the proapoptotic protein Bax (BCL2-associated X protein). TOMM22 interacts with 3-β hydroxysteroid dehydrogenase 2 (3βHSD2) and converts pregnenolone-to-progester |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Translocase of outer mitochondrial membrane 22 (TOMM22) is an essential receptor of the mitochondrial import channel. It is characterized with an N-terminal negatively charged region exposed to the cytosol, a putative transmembrane region and a C-terminal intermembrane space region with little negative charge. |
| Immunogen: | membrane fraction from Vero (monkey kidney-derived) cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |