Trypsin inhibitor from Glycine max (soybean)
SIGMA/T6522 - powder, BioReagent, suitable for cell culture
Synonym: SBTI
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082103
Product Type: Chemical
biological source | Glycine max (soybean) |
concentration | 10 mg/mL |
form | powder |
mol wt | 20.1 kDa |
product line | BioReagent |
Quality Level | 300 ![]() |
shipped in | ambient |
solubility | balanced salt solution: 1 mg/mL |
serum-free medium: soluble | |
specific activity | (One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.) |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
type | Type I-S |
Analysis Note: | One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein. |
Application: | This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin. |
Biochem/physiol Actions: | This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent. |
Components: | The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. |
Packaging: | 1, 5 g in glass bottle |
Packaging: | 25, 100, 5×100, 250, 500 mg in glass bottle |
Preparation Note: | The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C. |
Unit Definition: | One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginin |
Unit Definition: | One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H317 - H334 |
Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
Hazard Codes | Xn |
Risk Statements | 42/43 |
Safety Statements | 22-36/37-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: (One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.) |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |