Anti-γ-Tubulin antibody, Mouse monoclonal
SIGMA/T6557 - clone GTU-88, ascites fluid
Synonym: Anti-γ-Tubulin antibody, Mouse monoclonal
MDL Number: MFCD00677366
Product Type: Chemical
Western Blotting JOURNAL CITATION: Epstein-barr virus latency in B cells leads to epigenetic repression and CpG methylation of the tumour suppressor gene Bim. By: Paschos, K., Smith, P., et al. in PLoS Pathog, 2009. PubMed ID: 19557159 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1000492), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: RNA silencing of Mcl-1 enhances ABT-737-mediated apoptosis in melanoma: role for a caspase-8-dependent pathway. By: Keuling, A. M., Felton, K. E., et al. in PLoS One, 2009. PubMed ID: 19684859 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0006651), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Epstein-barr virus latency in B cells leads to epigenetic repression and CpG methylation of the tumour suppressor gene Bim. By: Paschos, K., Smith, P., et al. in PLoS Pathog, 2009. PubMed ID: 19557159 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1000492), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence COS cells undergoing mitosis transfected with doublecortin-GFP (DCX) (green) were stained using Monoclonal Anti-γ-Tubulin, clone no. GTU-88 (Cat. No. T6557) with Anti-Mouse IgG-TRITC (Cat. No. T6258) (red) and DAPI (DNA, blue). From Robert McNeil, Pediatrics Department, Baylor College of Medicine, Houston, TX.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained 1:5,000 Monoclonal Anti-γ-Tubulin Clone: GTU-88 (Cat. No. T6557). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:10,000 Monoclonal Anti-γ-Tubulin Clone: GTU-88 (Cat. No. T6557). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. U87 3. COS7 4. P19 5. RAT2 6. CHO 7. MDBK 8. MDCK
Immunoblotting
Whole cell extract of chicken fibroblasts was separated on SDS-PAGE, blotted with Monoclonal Anti-γ-Tubulin (Cat. No. T6557) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase conjugate (Cat. No. A1293) and NBT/BCIP substrate.
(Lane A) Antibody dilution 1:20,000; (Lane B) Negative control (only secondary antibody).
Whole cell extract of chicken fibroblasts was separated on SDS-PAGE, blotted with Monoclonal Anti-γ-Tubulin (Cat. No. T6557) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase conjugate (Cat. No. A1293) and NBT/BCIP substrate.
(Lane A) Antibody dilution 1:20,000; (Lane B) Negative control (only secondary antibody).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | GTU-88, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 48 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, hamster, chicken, human, bovine, canine, Xenopus, mouse |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: 1:5,000-1:10,000 using HeLa cells |
| indirect ELISA: suitable | |
| western blot: 1:10,000 using cultured chicken fibroblast extract | |
| UniProt accession no. | P23258  |
| Application: | Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA. |
| Application: | Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining. Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA. |
| Biochem/physiol Actions: | γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain. Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | synthetic γ-tubulin peptide, conjugated to KLH |
| Physical form: | The product is provided as ascites fluid with 15 mM sodium azide as a preservative. |
| Specificity: | Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin. |
| Specificity: | The antibody recognizes an epitope located within the N-terminal region of γ-tubulin. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |