Thymidine Phosphorylase, recombinant from Escherichia coli
SIGMA/T6632 - recombinant, expressed in E. coli, buffered aqueous solution, ≥900 units/mL, 0.2 μm filtered
Synonym: Thymidine:orthophosphate deoxy-D-ribosyltransferase
CAS Number: 9030-23-3
MDL Number: MFCD00132426
Product Type: Chemical
concentration | ≥900 units/mL |
form | buffered aqueous solution |
Quality Level | 300 ![]() |
recombinant | expressed in E. coli |
sterility | 0.2 μm filtered |
storage temp. | 2-8°C |
UniProt accession no. | P07650 ![]() ![]() |
Application: | Thymidine phosphorylase has been used in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy. Thymidine phosphorylase has also been used in a study to investigate implications for the clinical efficacy of nucleoside analogues. |
Biochem/physiol Actions: | An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays. |
General description: | Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation. |
Physical form: | Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin |
Preparation Note: | Cloned from E. coli and produced in overexpressing E. coli |
Unit Definition: | One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 2.4.2.4 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |