Trizma® hydrochloride
SIGMA/T6666 - pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
EC Number: 214-684-5
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
absorption | ≤0.010 at 280 in H2O at 0.5 M |
≤0.015 at 260 in H2O at 0.5 M | |
anion traces | sulfate (SO42-): ≤0.005% |
application(s) | diagnostic assay manufacturing |
assay | ≥99.0% (titration) |
cation traces | Al: ≤0.0005% |
As: ≤0.0001% | |
Ba: ≤0.0005% | |
Bi: ≤0.0005% | |
Ca: ≤0.001% | |
Cd: ≤0.0005% | |
Co: ≤0.0005% | |
Cr: ≤0.0005% | |
Cu: ≤0.0005% | |
Fe: ≤0.0005% | |
K: ≤0.005% | |
Li: ≤0.0005% | |
Mg: ≤0.0005% | |
Mn: ≤0.0005% | |
Mo: ≤0.0005% | |
Na: ≤0.005% | |
Ni: ≤0.0005% | |
Pb: ≤0.0005% | |
Sr: ≤0.0005% | |
Zn: ≤0.0005% | |
form | powder |
ign. residue (900°C) | ≤0.2% (as SO4) |
impurities | Insoluble matter, passes filter test |
InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
loss | ≤0.2% loss on drying, 110°C |
mp | 150-152 °C |
pH | 3.5-5.0 (0.5 M in H2O) |
pKa (25 °C) | 8.1 |
product line | BioXtra |
Quality Level | 400 ![]() |
SMILES string | Cl.NC(CO)(CO)CO |
solubility | H2O: 0.5 M, clear, colorless |
useful pH range | 7.0-9.0 |
Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Easily compare specifications for Trizma HCl products with the Trizma HCl specification table ![]() |
Packaging: | 1 kg in poly bottle |
Packaging: | 50, 250 g in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.0% (titration) |
mp | 150-152 °C |
UNSPSC | 12352104 |