Tumor Necrosis Factor-α human
SIGMA/T6674 - TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: hTNF-α; TNF-α
CAS Number: 94948-59-1
MDL Number: MFCD00148692
Product Type: Chemical
assay | ≥97% (SDS-PAGE) |
biological source | human |
form | powder |
impurities | <1 EU/μg |
mol wt | ~17.4 kDa |
packaging | pkg of 10 μg |
pkg of 5x10 μg | |
potency | 0.02-0.3 ng/mL ED50/EC50 |
quality | endotoxin tested |
Quality Level | 200 ![]() |
recombinant | expressed in E. coli |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P01375 ![]() ![]() |
Analysis Note: | The biological activity of TNF-α is measured by the cytolysis of murine L929 cells. |
Application: | TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production. |
Application: | Tumor Necrosis Factor-α (TNFα) human has been used: • to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells • to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression • to induce death-receptor-mediated apoptosis in HeLa cells. |
Biochem/physiol Actions: | Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated. |
Physical form: | Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose. |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
Purity | ≥97% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |