Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat
SIGMA/T6778 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Rabbit IgG (whole molecule)
MDL Number: MFCD00162790
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:400 Anti-Rabbit IgG (whole molecule)-TRITC antibody produced in Goat (Cat. No. T6778).
Immunofluorescence : SCK-CEN Mouse cortex primary neurons cultured for 10 days were stained for PSD95, Β-Tubulin III and nuclei. For PSD95 Monoclonal Anti-PSD95 antibody produced in Mouse (Cat. No. P246) and Anti-Mouse IgG (whole molecule)–FITC antibody produced in Goat (Cat. No. F2012) (green) were used. For Β-Tubulin III Anti-Β-Tubulin III antibody produced in Rabbit (Cat. No. T2200) and Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in Goat (Cat. No. T6778) (red). Nuclei were stained with Hoechst B2883 (blue/purple). Submitted by Dr G. Pani, Studiecentrum voor Kernenergie-Centre d′Etude d′Energie Nucleaire
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | TRITC conjugate |
| contains | ≥15 mM sodium azide as preservative |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 1:400 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 |
| Application: | Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry. |
| Application: | Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques. |
| Biochem/physiol Actions: | IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Binds all rabbit Igs. |
| General description: | IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds. |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |