Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse
SIGMA/T6793 - clone 6-11B-1, ascites fluid
Synonym: Anti-ALS22; Anti-H2-ALPHA; Anti-TUBA1
MDL Number: MFCD00164512
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Planar cell polarity enables posterior localization of nodal cilia and left-right axis determination during mouse and Xenopus embryogenesis. By: Antic, D., Stubbs, J. L., et al. in PLoS One, 2010. PubMed ID: 20126399 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0008999), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Exploring the transcriptome of ciliated cells using in silico dissection of human tissues. By: Ivliev, A. E., ′t Hoen, P. A., et al. in PLoS One, 2012. PubMed ID: 22558177 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0035618), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Neuroanatomy of the vestimentiferan tubeworm Lamellibrachia satsuma provides insights into the evolution of the polychaete nervous system. By: Miyamoto, N., Shinozaki, A., et al. in PLoS One, 2013. PubMed ID: 23372830 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055151), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Nerve pathways in the embryonic Zebrafish brain and spinal cord revealed by Monoclonal Anti-Acetylated Tubulin (clone no. 6-11B-1, Cat. No. T6793). Immunoperoxidase staining of a whole-mount embyro at 24 hours of development. From Dr. F. Zie and Prof. H. Reichert, Institute of Zoology, University of Basel.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:2,000 Monoclonal Anti-Tubulin, Acetylated Clone: 6-11B-1 (Cat. No. T6793). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:2,000 Monoclonal Anti-Acetylated Tubulin Clone: 6-11B-1 (Cat. No. T6793). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. U87 3. COS7 4. P19 5. RAT2 6. CHO 7. MDBK 8. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 6-11B-1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, protista, invertebrates, hamster, plant, frog, rat, monkey, pig, bovine, chicken, human |
| storage temp. | −20°C |
| technique(s) | dot blot: suitable |
| electron microscopy: suitable | |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| radioimmunoassay: suitable | |
| western blot: 1:2,000 using a bovine or rat brain preparation | |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse has been used in following studies: • immunofluorescence. • western blotting. • immunocytochemistry. • immunohistochemistry. |
| Biochem/physiol Actions: | Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000. |
| Immunogen: | acetylated α-tubulin from the outer arm of sea urchin sperm axonemes. |
| Specificity: | The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |