pPolh-FLAG™-1 Transfer Vector
SIGMA/T6824
Synonym: ddddk tag; dykddddk tag
Product Type: Chemical
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | pPolh-FLAG-1 is a 5575 bp baculovirus transfer vector used for producing FLAGÒ fusion proteins in insect cells. The vector is designed to be compatible with most baculoviral DNA systems that require the essential gene ORF1629 for complementation of lethal deletions and the recovery of viable recombinant virus. It is also a transfer vector for generation of recombinant Baculovirus and expression of N-terminal FLAG® fusions in insect cells. |
| General description: | The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome. |
| Legal Information: | FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | pPolh-FLAG is a trademark of Sigma-Aldrich Co. LLC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |