Thrombin from human plasma
SIGMA/T7009 - lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280 , 18.3)
Synonym: Factor IIa
CAS Number: 9002-04-4
EC Number: 232-648-7
MDL Number: MFCD00082072
Product Type: Chemical
Prothrombin is cleaved in vivo by activated factor X releasing the activation peptide and cleaving thrombin into light and heavy chains yielding catalytically active α-thrombin. α-Thrombin is composed of a light chain (A chain)(MW ~6,000) and a heavy chain (B chain)(~31,000). These two chains are joined by one disulfide bond.
| application(s) | diagnostic assay manufacturing |
| form | lyophilized powder |
| impurities | HIV and HBsAg, source material tested negative |
| mol wt | 37.4 kDa |
| Quality Level | 200  |
| specific activity | ≥1,000 NIH units/mg protein (E |
| storage temp. | −20°C |
| UniProt accession no. | P00734  |
| Analysis Note: | The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
| Application: | Thrombin from human plasma has been: • dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production • to polymerize fibrin on well plates • used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells |
| Biochem/physiol Actions: | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| Biochem/physiol Actions: | Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500. |
| General description: | The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2. |
| Other Notes: | View more information on thrombin at www.sigma-aldrich.com/enz. |
| Physical form: | Lyophilized from saline sodium citrate buffer, pH 6.5 |
| Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 NIH units/mg protein (E |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |