N,N,N′,N′-Tetramethylethylenediamine
SIGMA/T7024 - BioReagent, for molecular biology, ≥99% (GC)
Synonym: 1,2-
CAS Number: 110-18-9
Empirical Formula (Hill Notation): C6H16N2
Molecular Weight: 116.20
EC Number: 203-744-6
MDL Number: MFCD00008335
Linear Formula: (CH3)2NCH2CH2N(CH3)2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T7024-25ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T7024-50ML
| assay | ≥99% (GC) |
| bp | 120-122 °C (lit.) |
| density | 0.775 g/mL at 20 °C (lit.) |
| expl. lim. | 9.08 % |
| foreign activity | DNase, RNase, protease, none detected |
| grade | for molecular biology |
| InChI | 1S/C6H16N2/c1-7(2)5-6-8(3 |
| InChI key | KWYHDKDOAIKMQN-UHFFFAOYSA |
| mp | −55 °C (lit.) |
| product line | BioReagent |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | CN(C)CCN(C)C |
| Application: | This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases. TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,, The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules. |
| Packaging: | 25, 50, 100 mL in glass bottle |
| Quantity: | Suggested use is 35 μl for 50 ml acrylamide gel solution. |
| Symbol |    GHS02,GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H225 - H301 + H331 - H314 |
| Precautionary statements | P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | F,C |
| Risk Statements | 11-20/22-34 |
| Safety Statements | 16-26-36/37/39-45 |
| RIDADR | UN 2372 3 / PGII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 61.7 °F - closed cup |
| Flash Point(C) | 16.5 °C - closed cup |
| Purity | ≥99% (GC) |
| bp | 120-122 °C (lit.) |
| mp | −55 °C (lit.) |
| Density | 0.775 g/mL at 20 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12352200 |