Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride
SIGMA/T7254 - ≥96% (TLC), powder
Synonym: (3S)
CAS Number: 4272-74-6
Empirical Formula (Hill Notation): C14H21ClN2O3S · HCl
Molecular Weight: 369.31
EC Number: 224-266-4
MDL Number: MFCD00065395
Linear Formula: C14H21ClN2O3S · HCl
Product Type: Chemical
| assay | ≥96% (TLC) |
| biological source | synthetic (organic) |
| form | powder |
| InChI | 1S/C14H21ClN2O3S.ClH/c1-1 |
| InChI key | YFCUZWYIPBUQBD-ZOWNYOTGSA |
| potency | 10-100 μM effective concentration |
| Quality Level | 200  |
| SMILES string | Cl[H].Cc1ccc(cc1)S(=O)(=O |
| solubility | H2O: 50 mg/mL |
| storage temp. | −20°C |
| Application: | Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used: • in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract • as a protease inhibitor in tissue and cell homogenization • as a trypsin inhibitor to determine the specific protease activity levels |
| Biochem/physiol Actions: | Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. |
| Caution: | Stable at least two years when stored desiccated at −20°C. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | UN 3335 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% (TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |