Trizma® Pre-set crystals
SIGMA/T7443 - BioPerformance Certified, pH 7.2, average Mw 153.8
Synonym: Tris crystals
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T7443-100G
This image depicts SKU: T7443-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T7443-250G
This image depicts SKU: T7443-250G
| application(s) | diagnostic assay manufacturing |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | DNase, RNase, Protease, NICKase, none detected |
| endotoxin, tested | |
| total aerobic microbial count, tested | |
| ≤0.05 A290 | |
| ≤0.5% water (Karl Fischer) | |
| ≤5 ppm Heavy metals (as Pb) | |
| mol wt | average Mw 153.8 |
| pH | 7.2 |
| 7.20 (25 °C, 0.1 M)(lit.) | |
| Quality Level | 200  |
| solubility | water: 0.66 g/mL, clear, colorless |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7.0-9.0 |
| Application: | Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C. From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C. If a different concentration is desired, note that there is a concentration effect on the pH of the solution. |
| Application: | The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Certain pH electrodes do not give accurate results with Tris buffers. |
| Other Notes: | This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer. |
| Packaging: | 100, 250 g in poly bottle |
| Preparation Note: | Trizma solutions can be autoclaved. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |