Thrombin from bovine plasma
SIGMA/T7513 - lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
Synonym: Factor IIa
CAS Number: 9002-04-4
EC Number: 232-648-7
MDL Number: MFCD00166053
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| form | lyophilized powder |
| mol wt | heavy chain ~33 kDa |
| light chain ~5 kDa | |
| Quality Level | 200  |
| specific activity | ≥2,000 NIH units/mg protein (E |
| storage temp. | −20°C |
| UniProt accession no. | P00735  |
| Analysis Note: | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. |
| Analysis Note: | The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
| Biochem/physiol Actions: | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| Other Notes: | View more information on thrombin at www.sigma-aldrich.com/enz. |
| Physical form: | Lyophilized from saline sodium citrate buffer, pH 6.5 |
| Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 NIH units/mg protein (E |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |