Tris-Borate-EDTA buffer
SIGMA/T7527 - BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis
Synonym: TBE buffer
MDL Number: MFCD00236358
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
form | powder blend |
grade | for molecular biology |
impurities | DNase and RNase, none detected |
InChI | 1S/C10H16N2O8.C4H11NO3.BH |
InChI key | OSBLTNPMIGYQGY-UHFFFAOYSA |
pH | 8.1-8.5 (5 ×) |
product line | BioReagent |
Quality Level | 200 ![]() |
SMILES string | OB(O)O.NC(CO)(CO)CO.OC(=O |
solubility | water: 85.1 g/L, clear, colorless |
suitability | suitable for electrophoresis |
Application: | Ready for use in gel electrophoresis after dilution to working concentrations. |
Application: | TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
Packaging: | 1, 4 L in poly bottle |
Packaging: | 1L poly bottle contains a powder blend that can be dissolved and reconstituted within the bottle to prepare one liter of a 5× concentrate. 4L poly bottle contains a powder blend that can be dissolved and reconstituted to prepare four liters of a 5× concentrate. |
Preparation Note: | Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134). |
Reconstitution: | Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H360FD |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 60-61-36/37/38 |
Safety Statements | 53-26-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |