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Tris-Borate-EDTA buffer

SIGMA/T7527 - BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Synonym: TBE buffer

MDL Number: MFCD00236358
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T7527-1L 1 L
 $28.00
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45-T7527-4L 4 L
 $70.70
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This image depicts SKU: T7527-4L

 

application(s) diagnostic assay manufacturing
form powder blend
grade for molecular biology
impurities DNase and RNase, none detected
InChI 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key OSBLTNPMIGYQGY-UHFFFAOYSA-N
pH 8.1-8.5 (5 ×)
product line BioReagent
Quality Level 200 
SMILES string OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
solubility water: 85.1 g/L, clear, colorless
suitability suitable for electrophoresis
Application: Ready for use in gel electrophoresis after dilution to working concentrations.
Application: TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging: 1, 4 L in poly bottle
Packaging: 1L poly bottle contains a powder blend that can be dissolved and reconstituted within the bottle to prepare one liter of a 5× concentrate.
4L poly bottle contains a powder blend that can be dissolved and reconstituted to prepare four liters of a 5× concentrate.
Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Reconstitution: Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.
UNSPSC 12161700

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