Tumor Necrosis Factor-α from mouse
SIGMA/T7539 - TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: mTNF-α; TNF-α
MDL Number: MFCD00164823
Product Type: Chemical
SDS-PAGE Tumor Necrosis Factor-α from Mouse (Cat. No. T7539) was assessed on SDS-PAGE.
TNF alpha Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Bioassay for Tumor Necrosis Factor α The biological activity of mouse recombinant TNF-α (Cat. No. T7539) is measured by the cytolysis of murine L929 cells, a TNF-α sensitive mouse fibrosarcoma line. The EC50 is defined as the effective concentration of growth factor that elicits 50% inhibition of cell growth.
| assay | ≥95% |
| biological source | mouse |
| color | white |
| form | powder |
| impurities | ≤1 EU/μg protein endotoxin |
| mol wt | 17 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | 0.01-0.5 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P06804  |
| Application: | Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used: • to activate MS1 endothelium cells • to induce lipolysis in adipocytes • to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation |
| Biochem/physiol Actions: | Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated. |
| Physical form: | Lyophilized from 10 mM sodium phosphate, pH 7.5 |
| Unit Definition: | One unit is the amount required to induce half-maximal cytolysis of L929 cells. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |