Thrombin from human plasma
SIGMA/T7572 - 400-1000 NIH units/mg protein
Synonym: Factor IIa
CAS Number: 9002-04-4
EC Number: 232-648-7
MDL Number: MFCD00082072
Product Type: Chemical
form | lyophilized powder |
impurities | HIV, hepatitis B and hepatitis C, tested negative |
Quality Level | 200 ![]() |
specific activity | 400-1000 NIH units/mg protein |
storage temp. | −20°C |
UniProt accession no. | P00734 ![]() ![]() |
Analysis Note: | The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
Application: | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles. |
Biochem/physiol Actions: | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
General description: | Lyophilized powder containing sucrose, sodium chloride and Tris. |
General description: | Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. |
Other Notes: | View more information on thrombin ![]() ![]() |
Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H315-H319-H334-H335 |
Precautionary statements | P261-P305 + P351 + P338-P342 + P311 |
Hazard Codes | Xn,Xi |
Risk Statements | 36/37/38-42 |
Safety Statements | 22-24-26-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | 2 |
activity | specific activity: 400-1000 NIH units/mg protein |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 3.4.21.5 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |