Anti-TGN46 antibody produced in rabbit
SIGMA/T7576 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-MGC14722; Anti-TGN38 homolog; Anti-TGN48; Anti-TGN51; Anti-TGOLN2; Anti-TTGN2; Anti-Trans-Golgi network integral membrane protein 2 precursor; Anti-Trans-Golgi network protein TGN51; Anti-Trans-golgi network protein 2
MDL Number: MFCD09265378
Product Type: Chemical
Indirect Immunofluorescence Human A549 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Anti-TGN46 antibody (Cat. No. T7576). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
Enhanced Validation-By Recombinant Expression Western Blotting. Antibody recognition: Anti-TGN46 antibody produced in Rabbit (Cat. No. T7576) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing TGN46 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 0.5 μg/mL.
Immunofluorescence A549 human lung carcinomacells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 10 μg/mL Anti-TGN46 antibody produced in Rabbit (Cat. No. T7576). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of HEK-293T cells transfected with Human TGN46 were separated on SDS-PAGE and probed with Anti-TGN46 antibody produced in Rabbit (Cat. No. T7576). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.5 μg/mL antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 80-100 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 10-20 μg using TGN46 from human HepG2 cell lysates. |
| indirect immunofluorescence: 5-10 μg/mL using human A549 cells | |
| western blot (chemiluminescent): 0.25-0.5 μg/mL using extracts of human HEK-293T cells expressing recombinant human TGN46 | |
| UniProt accession no. | O43493  |
| Application: | Rabbit polyclonal anti-TGN46 antibody is used to tag trans-golgi network protein 2 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of trans-golgi network protein 2 as a membrane traffic regulating component of the trans-Golgi network (TGN). Anti-TGN46 may be used as a TGN marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-TGN46 recognizes human trans-golgi network protein 2 (TGN46). Detection of the TGN46 band by immunoblotting is specifically inhibited by the immunizing peptide. |
| General description: | TGN46 (Trans-Golgi network protein, 46 kDa), the human homologue of rat TGN38, is a resident integral membrane protein of the trans-Golgi network (TGN) that cycles constitutively between the TGN and the plasma membrane, returning via endosomes. The TGN is the major sorting compartment of the secretory pathway for proteins, lipids and membrane traffic. TGN46 is a heavily glycosylated protein, probably involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. The membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively for localization in the TGN. TGN46 is widely expressed. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 426-437 of human TGN46 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in monkey and differs by three amino acids in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |