Thunderbolt™ GC10™ Electrocompetent Cells
SIGMA/T7699 - for generation of cDNA libraries and DNA plasmid production
MDL Number: MFCD05665590
Product Type: Chemical
Representative product in the box
Representative product in the bag
| form | suspension |
| grade | for molecular biology |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Suitable for recovery of high quality plasmid DNA and generation of cDNA libraries from plasmid-based vectors |
| Components: | • Thunderbolt GC10 electrocompetent cells, 5 X 80 μL or 5 X 100 μL (T7074) • pUC 19 control DNA (10 ng/μL), 10 μL (D2567) |
| Features and Benefits: | • Ensures recovery of stable, high quality plasmid DNA as well as methylated DNA • Renders protection to clonal stocks from T1 and T5 bacteriophage contamination • Allows for β-galactosidase α-complementation for blue/white screening • Guaranteed high transformation efficiency • Convenient 80 μL or 100 μL aliquots |
| General description: | Thunderbolt GC10 electrocompetent E. coli have a very high transformation efficiency of >1x1010 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA. They are comparable to DH10β™ strain. |
| Legal Information: | DH10β is a trademark of Invitrogen Corp. |
| Legal Information: | GC10 is a trademark of GeneChoice, Inc. |
| Legal Information: | Thunderbolt is a trademark of GeneChoice, Inc. |
| Principle: | Thunderbolt GC10 electrocompetent E. coli is K strain bacteria that contain mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5. The host restriction systems are eliminated to allow the cloning of methylated DNA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 1 |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |