Thimet Oligopeptidase from Bacillus licheniformis
SIGMA/T7705
Product Type: Chemical
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| form | powder |
| mol wt | ~77 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | Thimet oligopeptidase can be used for the degradation of collagen in combination with collagenases. It can also be used for the hydrolysis of neuropeptides such as bradykin, neurotensin, and amyliod-β-peptide. Thimet oligopeptidase has been used in a study to investigate the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry: ventral striatum, hippocampus, and frontal cortex. |
| General description: | Thimet oligopeptidase is considered essential in the degradation of collagen in collaboration with collagenolytic enzymes. Thimet oligopeptidase (TOP) is a neuropeptidase involved in the hydrolysis of gonadotropin-releasing hormone, a key component of the hypothalamic-pituitary-go |
| Preparation Note: | This enzyme has been affinity chromatographically purified using a niquel affinity column. It contains a 6-Histidine tag in its C-terminus. A working solution of this enzyme can be prepared in 20 mM phosphate buffered saline solution, pH 7.0, or sterile and deionized water, pH 7.0. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H332 |
| Hazard Codes | Xn |
| Risk Statements | 20-38 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.15 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |