Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat
SIGMA/T7782 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162624
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:800 Anti-Mouse IgG (Fab specific)-TRITC antibody produced in Goat (Cat. No. T7782).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:100 Monoclonal Anti-β2-Microglobulin antibody produced in Mouse, Clone: BM-63 (Cat. No. M7398) followed by 1:32 Anti-Mouse IgG (Fab specific)-TRITC antibody produced in Goat (Cat. No. T7782).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | TRITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:64 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Conjugation of TRITC to Mouse IgG (Fab specific) antibody enables the use in immunofluorescent techniques. |
| Biochem/physiol Actions: | IgG antibody subtype provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) antibody participates in complement fixation and opsonization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG)antibody is found in four subclasses IgG1, IgG2, IgG3 and IgG4. It has a monomeric structure. IgG is secreted by B cells and is found in blood and extracellular fluids.. Anti-Mouse IgG (Fab specific) is conjugated to tetramethylrhodamine isothiocyanate (TRITC) and then purified by gel filtration to remove free TRITC. |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse or human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |