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Trizma® Pre-set crystals

SIGMA/T7818 - BioPerformance Certified, pH 7.5, average Mw 150.6

Synonym: Tris crystals

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T7818-100G 100 g
 $138.00
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45-T7818-250G 250 g
 $290.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: T7818-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: T7818-250G

 

application(s) diagnostic assay manufacturing
form crystalline powder
grade BioPerformance Certified
impurities DNase, RNase, Protease, NICKase, none detected
  endotoxin, tested
  total aerobic microbial count, tested
  ≤0.05 A290
  ≤0.5% water (Karl Fischer)
  ≤5 ppm Heavy metals (as Pb)
mol wt average Mw 150.6
pH 7.5
  7.50 (25 °C, 0.1 M)(lit.)
Quality Level 200 
solubility water: 0.66 g/mL, clear, colorless
technique(s) cell culture | mammalian: suitable
useful pH range 7.0-9.0
Application: Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Application: The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Certain pH electrodes do not give accurate results with Tris buffers.
Other Notes: This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Packaging: 100, 250 g in poly bottle
Preparation Note: Trizma solutions can be autoclaved.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
UNSPSC 12161700

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800-334-7725
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