Thermolysin from Geobacillus stearothermophilus
SIGMA/T7902 - powder, BioReagent, 30-350 units/mg protein (E1%/280 ), suitable for cell culture
Synonym: Protease from Geobacillus stearothermophilus; Thermophilic-
CAS Number: 9073-78-3
EC Number: 232-973-4
MDL Number: MFCD00165549
Product Type: Chemical
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| biological source | Geobacillus stearothermophilus |
| form | powder |
| mol wt | 34.6 kDa |
| product line | BioReagent |
| purified by | crystallization |
| Quality Level | 200  |
| specific activity | 30-350 units/mg protein (E |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| single cell analysis: suitable |
| Application: | A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 100, 250 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Physical form: | lyophilized powder containing calcium and sodium acetate buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42/43 |
| Safety Statements | 22-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 30-350 units/mg protein (E |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.27 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |