Transforming Growth Factor-α human
SIGMA/T7924 - TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: hTGF-α; TGF-α
CAS Number: 105186-99-0
Empirical Formula (Hill Notation): C239H348N70O72S6
Molecular Weight: 5546.14
MDL Number: MFCD00082077
Linear Formula: C239H348N70O72S6
Product Type: Chemical
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| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | human |
| form | powder |
| impurities | ≤1 EU/μg |
| mol wt | 5.5 kDa |
| packaging | pkg of 1 mg |
| pkg of 5 mg | |
| potency | ≤0.2 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01135  |
| Amino Acid Sequence: | Val-Val-Ser-His-Phe-Asn-A |
| Analysis Note: | The biological activity is measured by its ability to stimulate 3H-thymidine incorpation into BALB/c 3T3 cells. |
| Application: | Transforming Growth Factor-α human has been used: • to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles • in human hepatocytes cell culture and keratinocyte cell culture • to induce morphogenesis in primary mouse organoids |
| Biochem/physiol Actions: | The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy. |
| Biochem/physiol Actions: | Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor. |
| General description: | The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33. TNFα is a member of TNF superfamily. |
| Physical form: | Lyophilized from a 0.2 μm filtered buffered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |