Monoclonal Anti-β-Tubulin IV antibody produced in mouse
SIGMA/T7941 - clone ONS.1A6, ascites fluid
Synonym: Anti-DYT4; Anti-TUBB4; Anti-beta-5
MDL Number: MFCD02095891
Product Type: Chemical
Immunoblotting MCF7 Human breast carcinoma cells extract was separated on SDS-PAGE and probed with Monoclonal AB To β Tubulin IV CL:ONS.1A6 (Cat. No. T7941). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/400.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ONS.1A6, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | hamster, chicken, human, bovine, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:400 using cultured human breast adenocarcinoma cell line MCF-7 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Immunofluorescence (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-β-Tubulin IV antibody has been used in immunostaining. |
| Application: | Monoclonal Anti-β-Tubulin IV antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections), indirect ELISA, microarray and western blot (1:400 using cultured human breast adenocarcinoma cell line MCF-7). The product may also be used for immunocytochemistry and immunofluorescence . |
| Biochem/physiol Actions: | β-Tubulin IV creates the oligodendrocyte microtubule network, that helps in the transport of myelin proteins, lipids and RNA at the time of myelination. It participates in the generation of early fibrotic stages. TUBB4 plays a major role in the transport and proper localization of N-cadherin within the plasma membrane. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Tubulin IV codes for β-tubulin 4A (TUBB4A). TUBB4A is expressed in the central nervous system by oligodendrocytes. It is located on human chromosome 19p13.3. |
| General description: | Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have four different isotypes in brain cells, ranging from β I to β IV . Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Monoclonal Anti-β-Tubulin IV antibody is specific for epitope located in the C-terminal sequence of β-tubulin isotype IV in humans, bovines, hamsters, rats, mice and chickens. The product does not react with other tubulin isotypes. |
| Immunogen: | synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |