Tau Protein Ladder, 6 isoforms human

SIGMA/T7951 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

MDL Number: MFCD09265460
Product Type: Chemical

assay	≥90% (SDS-PAGE)
biological source	human
form	buffered aqueous glycerol solution
Quality Level	200
recombinant	expressed in E. coli
shipped in	dry ice
storage temp.	−20°C
technique(s)	immunoelectrophoresis: 10-20 μL using recombinant Tau protein marker
	western blot: 2-5 μL using recombinant Tau protein marker
UniProt accession no.	P10636

Application:	Tau Protein Ladder, 6 isoforms human has been used as a sample in immunomagnetic reduction (IMR) assay. It has also been used as positive control in western blotting.
Biochem/physiol Actions:	In neurons, tau protein plays an important role in maintaining microtubule assembly and stability. It acts as a connector between microtubules and other cytoskeletal elements or proteins. Overexpression of the protein has been observed in Alzheimer′s disease and various neurodegenerative disorders denoted as ‘tauopathies′. Thus, pathological tau proteins acts as a potential biomarker the neurodegenerative process.
General description:	Tau gene, spanning 100 kb with 16 exons, is mapped to human chromosome 17q21. The six alternative splice variants of protein ranging in size from 352-441 amino acids have been identified in human adult brain. Tau is a member of the microtubule-associated protein (MAP) family. It is predominantly expressed in neurons.
Packaging:	A 50 μL vial of Tau Protein Ladder contains 0.25 μg of each of the six isoforms.
Physical form:	Solution in 125 mM Tris-HCl, pH 6.8, containing 4% SDS, 10% 2-mercaptoethanol, 20% glycerol, and 0.004% bromphenol blue.
Specifications:	Contains 6 recombinant Tau proteins expressed in E. coli, which comprise six Tau isoforms with molecular weights of 45,900, 42,600, 42,900, 39,700, 40,000, and 36,800 respectively. No histidine-tags are present in the proteins.