Anti-TRB3 (N-terminal) antibody produced in rabbit
SIGMA/T8076 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Human tribbles 3 homolog; Anti-NIPK; Anti-SINK; Anti-SKIP3; Anti-TRIB3
MDL Number: MFCD08705525
Product Type: Chemical
Western Blotting JOURNAL CITATION: Smyd1 facilitates heart development by antagonizing oxidative and ER stress responses. By: Rasmussen, T. L., Ma, Y., et al. in PLoS One, 2015. PubMed ID: 25803368 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0121765), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Smyd1 facilitates heart development by antagonizing oxidative and ER stress responses. By: Rasmussen, T. L., Ma, Y., et al. in PLoS One, 2015. PubMed ID: 25803368 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0121765), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting
Cell lysates were separated on SDS-PAGE and blotted with Anti-TRB3 (N-terminal) (Cat. No. T8076). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Antibody dilution 1:1,000
Lanes
1: HEK293 cells transfected with human TRB3
2: HEK293 cells (untransfected)
3: HEK293 cells transfected with human TRB3; Inhibition of antibody binding with TRB3 immunizing peptide (human 11-29) (20 μg/mL)
Cell lysates were separated on SDS-PAGE and blotted with Anti-TRB3 (N-terminal) (Cat. No. T8076). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Antibody dilution 1:1,000
Lanes
1: HEK293 cells transfected with human TRB3
2: HEK293 cells (untransfected)
3: HEK293 cells transfected with human TRB3; Inhibition of antibody binding with TRB3 immunizing peptide (human 11-29) (20 μg/mL)
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-TRB3 (N-terminal) antibody produced in Rabbit (Cat. No. T8076) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing TRB3 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 39 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293 cells expressing human TRB3 |
| UniProt accession no. | Q96RU7  |
| Application: | Anti-TRB3 (N-terminal) antibody produced in rabbit has been used in immunoprecipitation and immunoblotting. |
| Biochem/physiol Actions: | Tribbles homolog 3 (TRB3) like tribbles has also been identified as a potential pro-apoptotic protein, and is induced by a variety of cell stress conditions, including hypoxia and nutrient starvation. It was previously identified as a neuronal cell death-inducible protein kinase (NIPK) that is rapidly upregulated during neuronal apoptosis. TRB3 binds to activating transcription factor 4 (ATF4) and inhibits its transcriptional activity. TRB3 has been shown to interact with mitogen-activated protein kinases (MAPK) kinases to regulate activation of MAPK pathways. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tribbles homolog 3 (TRB3) gene is located on the 20p13 human chromosome. TRB3 and its related family members TRB1 and TRB2 show 45% sequence identity. It exhibits resemblance to ′tribbles′, a drosophila protein. TRB family members have a truncated kinase domain that lacks an adenosine 5′-triphosphate binding site (GXGXXG) and contain a variant catalytic core motif. |
| Immunogen: | synthetic peptide corresponding to amino acids 11-29 located near the N-terminus region of human TRB3. The immunizing sequence has limited homology (~50% identity) to mouse and rat TRB3, and has no identity to TRB1 and TRB2 isoforms. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |