Trypan Blue solution
SIGMA/T8154 - 0.4%, liquid, sterile-filtered, suitable for cell culture
Synonym: Blue cell viability dye
CAS Number: 72-57-1
Empirical Formula (Hill Notation): C34H24N6Na4O14S4
Molecular Weight: 960.81
MDL Number: MFCD00003969
Linear Formula: C34H24N6Na4O14S4
Product Type: Chemical
application(s) | cell analysis |
concentration | 0.4% |
form | liquid |
InChI | 1S/C34H28N6O14S4.4Na/c1-1 |
InChI key | GLNADSQYFUSGOU-UHFFFAOYSA |
Quality Level | 200 ![]() |
shipped in | ambient |
SMILES string | [Na+].[Na+].[Na+].[Na+].C |
sterility | sterile-filtered |
storage condition | dry at room temperature |
technique(s) | cell culture | mammalian: suitable |
tissue processing: suitable |
Application: | Trypan Blue solution has been used: • in cell viability assay to count viable and dead cells • in rescue assay to count viable cells • in trypan blue exclusion counting method to determine proliferation curve • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability • to count GEC cells using a hemocytometer |
General description: | Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells. |
General description: | Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method. |
Preparation Note: | Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H350 |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 45 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12352200 |