Trypan Blue solution

SIGMA/T8154 - 0.4%, liquid, sterile-filtered, suitable for cell culture

Synonym: Blue cell viability dye

CAS Number: 72-57-1
Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.81
MDL Number: MFCD00003969
Linear Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-T8154-20ML	20 mL	$10.80 1/EA		Add To Favorites
45-T8154-100ML	100 mL	$36.20 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

application(s)	cell analysis
concentration	0.4%
form	liquid
InChI	1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key	GLNADSQYFUSGOU-UHFFFAOYSA-J
Quality Level	200
shipped in	ambient
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
sterility	sterile-filtered
storage condition	dry at room temperature
technique(s)	cell culture \| mammalian: suitable
	tissue processing: suitable

Application:	Trypan Blue solution has been used: • in cell viability assay to count viable and dead cells • in rescue assay to count viable cells • in trypan blue exclusion counting method to determine proliferation curve • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability • to count GEC cells using a hemocytometer
General description:	Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
General description:	Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
Preparation Note:	Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

Symbol	GHS08
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	45
Safety Statements	53-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

12352200

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