Transferrin human
SIGMA/T8158 - powder, BioReagent, suitable for cell culture
Synonym: Siderophilin, partially saturated
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL Number: MFCD00132442
Product Type: Chemical
| assay | ≥98% |
| concentration | ~25 mM |
| form | powder |
| impurities | endotoxin, tested |
| hepatitis and HIV antibody, none detected | |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | H2O: 50 mg/mL |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02787  |
| Analysis Note: | Purity by agarose electrophoresis. |
| Application: | Transferrin human has been used: • to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity • as a supplement in serum-containing/devoid medium for human thyroid cell culture • as a target glycoprotein to develop an in-gel hydrazinolysis method |
| Biochem/physiol Actions: | Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form. |
| General description: | Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain. |
| Other Notes: | Partially iron-saturated |
| Preparation Note: | Heat treated at 60 °C for ten hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |