Anti-α-Tubulin antibody, Mouse monoclonal
SIGMA/T8203 - clone AA13, purified from hybridoma cell culture
Synonym: Anti-Tuba1a; Anti-Tubulin, α1A
MDL Number: MFCD00145891
Product Type: Chemical
Enhanced Validation-By Independent Antibodies HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:500 Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: DM1A (Cat. No. T9026) (A) and developed using Goat Anti-Mouse IgG, Cy3 conjugate, and 1:500 Anti-α-Tubulin antibody, Mouse monoclonal, Clone: AA13 (Cat. No. T8203) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate antibody. Results Two Anti-α-Tubulin antibodies, T9026 (A) And T8203 (B) target different regions of α-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Monoclonal Anti-α-tubulin Clone: AA13 (Cat. No. T8203). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Monoclonal Anti-α-tubulin Clone: AA13 (Cat. No. T8203). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-α-tubulin Clone: AA13 (Cat. No. T8203). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Neuro-2a 2. HeLa 3. HEK- 293T 4. Jurkat 5. Rat brain 6. COS7 7. NIH- 3T3 8. NRK 9. PC12
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AA13, monoclonal |
| concentration | ~2.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using Neuro-2a cell extract | |
| UniProt accession no. | Q71U36  |
| Application: | Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting. |
| Application: | The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry. |
| Biochem/physiol Actions: | Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS). |
| Biochem/physiol Actions: | Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin. |
| General description: | Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development. The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein. |
| Immunogen: | rat brain tubulin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |